based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. April 22 Asking Price: $15,990 USD Shoukat Mumtaz / Zantek Medical phone: +1 (952) 657-7977 (3). and FlexStation 3 Multi-Mode Microplate Readers have been validated for use with the ValitaTiter and ValitaTiter Plus assays. CAT activity was measured using SpectraMax M5/M5 Microplate Reader (Molecular Devices, MDS Analytical Technologies). The assay applies fluorescence polarization to quantify Fc-containing IgG. Conclusions We report a peptide-induced super-assembly of enzyme-containing MOFs for programmed enzyme. Cuvette port functionality can also be controlled using SoftMax Pro Software. For biological applications involving small samples, such as purificationThe guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. All associated StakMax® stackers. When a feature or capability applies to only certain readers, this exception is noted. When a feature or capability applies to only certain readers, this exception is noted. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). This reader's dual monochromators for. 6: bioNova científica, s. Background fluorescence has been subtracted. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Detection cartridge modularity allows for easy addition of new read modes within minutes without. All associated StakMax® stackers. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. 96-384. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The dried paper microwell plate was then placed in the SpectraMax M-5 microplate reader, and the signal intensities were measured for each well. Example: [Drawer]. The readings with excitation at 540 nm and emission at 600 nm represented the amount of phosphorylated IκB-α in the cells. • Tel. SpectraMax® M4. for ValitaTiter Plus standards read on Molecular Devices readers. Expanded wavelength coverage. Maximum signal with lowest background. A: Microplate Preparation. Recertification is recommended every two years. Figure 4: Transcreener ADP. Molecular Devices | Life Science Applications | Bioanalytical. Fluorescence from the Alamar blue® assay was detected by SpectraMax M5 microplate reader (Molecular Device Inc. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode…. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The cells were then treated to induce apoptosis. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The readings with excitation at 360 nm and emission at 450 nm represented the amount of. Cavicide (Warehouse, Cat # 79300360). book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. SpectraMax M3 M4 M5 M5e SpectraTest FL1 revA. SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E. 313 reference gain check fail. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. This application note discusses the principles on which PathCheck iswells in a microplate (all instruments) or individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Then, after H 2 O 2 was added, the fluorescence was measured for each time period at 488 nm (excitation) and 525 nm (emission) wavelengths on a SpectraMax M5 microplate reader. Sale price $16,495. We would like to show you a description here but the site won’t allow us. Table 3 shows the ratios obtained on all Molecular Devices plate readers tested for the minimum required assay control dilutions for each assay. Molecular Devices Corporation. Learn how to set up protocols, customize reports, export results, and more with this PDF document. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 00. Fresh cecum content samples were collected for 16S rRNA sequencing by Shanghai Majorbio Bio-Pharm Technology Co. The absorbance values at 450 nm were determined by the SpectraMax M5 microplate reader. If you do not have an iLab account you must first make one: The Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 95. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. 2 only). This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. book Page 1 Wednesday, July 7, 2010 2:09 PM The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. AquaMax® 4000. 00 USD. 7°C (gradient heating design). Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. 49 2. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. Small, unbound molecules rotate rapidly in. Call 516-939-0888 to check. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. Italic and boldface type are used for emphasis. SpectraMax M3, M4, M5 and M5e Multi-Mode Microplate Readers User Guide Fluorescence Microplate Reader Comparison Chart. E Models. LMax II 384 Microplate Luminometer: Integration time = 1 second, Settling time = off, Autoread = off. 00 USD. The cells were then treated to induce apoptosis. Then, 150 μL of dimethyl sulfoxide was added to each well and shaken gently for 10 min. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. SoftMax Pro Software has been integrated by many leading robotics andFor readers without a cuvette port, simply check the box next to ‘PathCheck’. 0 2. We use Spectramax M5 microplate reader in our lab. 2. The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. analysis, controls the SpectraMax Multi-Mode Microplate Readers. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. ) by nonlinear regression using. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. Validate the optical performance of your absorbance . Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. With optimized reagents, option to operate the readers. The. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. STAKMAX. Temperature control range from Ambient +4º to 50ºC. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. TR-FRET assay principle. 63 1. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. The cuvette must be in place when you read the plate. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. Fill a clean plate with water. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Both readers feature an 8-channel read head for fast reads and. 0. book Page 7 Wednesday, July 7, 2010 2:09 PM. Next, add 10 μl of the MTT labeling reagent (5 mg/mL) to each well for 4 h. temperature and fluorescence was measured at Ex/Em 490/585 nm using a Molecular Devices SpectraMax M5 microplate reader. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplateinstalled if you are using a SpectraMax ABS microplate reader **SoftMax Pro Software version 7. upgradeable with a wide range of high performance capabilities ideal for life science research and drug. Depending on which. SpectraMax microplate readers Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. Self Service Available. Spectramax M5 microplate reader: Molecular Devices: N/A:. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. Most papers they cultured bacteria to OD600>0. Equipped with scanning, shaking and kinetics modules. Between steps, reactions were rinsed 3 times with TBS + 0. Bottom-reading is usually recommended for cell-based assay with adherent cells. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. For luminescence, the SpectraMax M5 Microplate Reader utilizes a dedicated luminescence photomultiplier tube (PMT), providing the user the maximum signal and lowest background possible for glow luminescence reporter gene assays. Keywords. 4 0. Incubation. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence microplate readers. Synergy H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. Microplate reader settings. 3 or higher) SpectraMax® M5 and M5e Multi-Mode Microplate Readers; SpectraMax® M4 Multi-Mode Microplate Reader;. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Microbiota DNA was. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. Maximum signal with lowest background. Bookings are free, but you will need to use iLab to book. Urea-induced unfolding of rA1AT-WT and the Q9N mutant was measured using a SpectraMax. D. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. TNF standard curve. 96-1536. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. . Molecular Devices SpectraMax M5 ; Molecular Devices SpectraMax M2. IMAP FP KINASE ASSAYS ON THE SPECTRAMAX M5 MULTI-MODE MICROPLATE READER 校准标准品的制备 Table 1. Validating the absorbance optical performance of your microplate reader is as simple as reading a plate. Choose from a three- (M3), four- (M4), oron the SpectraMax Multi-Mode Microplate Readers by issuing a single. 2. Introduction. Add to Cart. When a The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. 1. Go to Product Details. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Figure 1. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. 72 MB. The assay window spanned four orders of magnitude. : 2 Oct 12 Page 2 of 10 Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The optical density (OD) was measured at 450 nm with a SpectraMax M5 microplate reader (Molecular Devices). An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD5 reader. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. SpectraMax Plus 384 (Molecular Devices) [4 units] UV and visible absorbance monochromator-based microplate reader SpectraMax M5 (Molecular Devices) [2 units] multi-label monochromator and filter-based microplate reader for UV and visible absorbance, fluorescence intensity, time-resolved fluorescence, fluorescence. No accessories are needed and SoftMax® Pro Software. The Spectr. We would like to show you a description here but the site won’t allow us. 0 . SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Example: [Drawer]. The reader uses two scanning monochromators to determine the optimal excitation and emission settings for the spectral characteristics of. Step 2. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. For readers without a cuvette port, these options are not shown in the software. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. Tunable wavelength selection / monochromator. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The dual monochromator optics allow the widest. SpectraMax M3, M4, M5, and M5 e Multi-Mode Microplate Readers 0112-0115 F 9 PathCheck ® Pathlength Meas urement Technology A SpectraMax Multi-Mode Microplate Reader with PathCheck Pathlength Measurement Technology allows normalization of variable well volumes to 1-cm cuvette readings. Wash Buffer Concentrate was diluted 10-fold. Five preferred detection modes. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual. Incubation to 70 o C enables high temperature assays such as enzyme kinetics, protein stability/aggregation. SpectraMax® ABS Plus. Learn how to set up protocols, customize reports, export results, and more with this PDF document. Microplate formats. Figure 1. Sensitive fluorescent quantitation of DNA with the Quant-iT. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideSpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. 6 for further experiment, so I'm confused which i would choose for OD value determination. This integrated system provides users with a multi-detection platform capable of increasing the liquid handling throughput and flexibility for biochemical- and cell-based kinetic assays. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. SpectraMax® ABS Plus. Published Dec 16, 2020 | Updated Dec 16, 2020. Lower limits of detection for ATP determined using the ATPlite . Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. 1 & 7. Self Service Available. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. It cannot be used for luminescence measurements. Make sure the microplate is flat against. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. Choose from a three- (M3), four-. The absorbance was measured at 490 nm using a SpectraMax® M5 microplate reader (Molecular Devices, USA). SpectraMax M Series Multi-Mode Microplate Readers 7 The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2µL samples with 24- or 64-well plates. Key applications: ELISA, nucleic acid, endotoxin testing. (Beijing, China) and a BCA protein assay kit from Beijing Solarbio Technology Co. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. Protocol Name Version 6. Bookings are free, but you will need to use iLab to book. Figure 1. SpectraMax M5 microplate reader (Molecular Devices, California, USA) with excitation at 450 nm and emission at 570 nm. Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). It measures the optical density (OD) of samples in a microplate or in a cuvette at aThe cells were then washed three times with PBS, and the fluorescent intensity of the cells was measured with a SpectraMax M5 microplate reader (Molecular Devices, USA) at an excitation wavelength of 488 nm and emission wavelength of 525 nm. Plate chamber design of the SpectraMax category 1 microplate readers. A: Microplate Preparation. "With this complete portfolio of multi-mode configurations, we. Microplate Readers Biotek Synergy H1 Hybrid Microplate. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s. The Perkin Elmer Wallac 1420 VICTOR2™ is a high performing microplate reader built for multi-tasking functions. Western blot analyses. If you do not have an iLab account you must first make one: Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. Table of Contents. iD5 i3x M5 Paradigm Delta mP 69 85 92 88 Average StDev (mP) 1. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. 6 for further experiment, so I'm confused which i would choose for OD value determination. FlexStation™ I. Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. Microplate Readers include the M3, M4, M5, and M5e models. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-,. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. The drawing shows a standard microplate of 15 mm height covered with a lid. HTRF-compatible SpectraMax Multi-Mode Readers. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. Examples: “Press carefully to engage,” “ Do not. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Read the Cuvette 1. 2. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. 5 12. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. Measurements of fluorescence and fluorescence polarization were performed on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA) with the excitation and emission wavelengths 544 nm and 590 nm, respectively. Microplate Capacity. com A. Flurescence intensities were read using a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Softmax Pro GxP Software 6. Five. both cuvette-based and microplate reader applications The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The absorbance was measured at 570 nm using a SpectraMax M5 microplate reader (Molecular Devices, Sunnyvale, CA). Three independent experiments were performed. Molecular Devices Spectramax M5 Microplate Reader; Related products. 306 plate air cal. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. SpectraMax Lmicroplate luminometer. PURPOSE. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Western blotting analysis. In brief, RAW 264. Note: this assay can also be detected using the luminescence mode of SpectraMax M5/M5 e and FlexStation 3multi-mode microplate readers. B: Selection of Excitation Wavelength. Wide range of applications. Qualify the fluorescence performance of the SpectraMax . The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Ltd. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. A serial dilution of IgG standards was performed, using XP Media/L-glutamine as the diluent, to concentrations from 2. • SpectraMax Mini Multi-Mode Microplate Reader (AccuBlue HiRange only) • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. M3-M5e_manual. S. Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit microplate reader. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 2. Download PDF. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never have to worry about not having the right set of filters. For a higher . Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The dual monochromator optics allow. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. Designed to provide the simplicity, flexibility and power required for advanced data analysis. 4. The SpectraMax® ABS Plus Microplate Reader can run . Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. Catalog Number: (89212-396) Supplier: Molecular Devices. or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. KEY FEATURES • Five. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. The following document is. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 67. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. System Diagrams and Dimensions. The guide also covers the software features, the specifications, and the accessories of the systems. Here, we illustrate how the SpectraMax iD5 reader, in combination with SoftMax ® Pro Software, is used to quantitate a cellular protein sample with two different kits, Pierce BCA Protein Assay Kit and Pierce Rapid. It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. Bookings are free, but you will need to use iLab to book. Preparation of the peritoneal macrophages The peritoneal macrophages were obtained from the mice after intraperitoneally (i. Learn how you can utilize microplate readers to drive landmark discoveries. Figure 3. (Shanghai, China). Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. Note: In this user guide, all references to SpectraMax Multi-Mode. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. 7°C and the bottom to 36. As readout, fluorescence was measured at Ex/Em = 540/590 nm using a SpectraMax M5 microplate reader with SoftMax Pro v7 (Molecular devices) after 4–6 h of incubation in normal cell-culture medium. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112. Detection of pro-inflammatory factorsThe SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. 1. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. , Sunnyvale, CA) using the bottom well-scan mode where nine readings. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. FlexStation® 3 Multi-Mode Microplate Reader. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Fill a clean plate with water. You can use either. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. Introduction. Recommended Optics Wavelength (nm) Wavelength. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. The dual monochromator optics allow the widest range of applications to be utilized for bior. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. 3. The Multiskan Sky microplate reader is an absorbance plate reader. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. While they play an important role in generating data from a wide range of. Wide range of applications. Turn on the microplate reader. SpectraMax® ABS. Lift up the lid over the chamber to insert or remove a cuvette. Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. SpectraMax M Series multi-mode microplates are configurable readers with triple.